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Phase imaging of moving DNA molecules and DNA molecules replicated in the atomic force microscope.

機(jī)譯:移動(dòng)的DNA分子和在原子力顯微鏡中復(fù)制的DNA分子的相位成像。

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摘要

Phase imaging with a tapping mode atomic force microscope (AFM) has many advantages for imaging moving DNA and DNA-enzyme complexes in aqueous buffers at molecular resolution. In phase images molecules can be resolved at higher scan rates and lower forces than in height images from the AFM. Higher scan rates make it possible to image faster processes. At lower forces the molecules are imaged more gently. Moving DNA molecules are also resolved more clearly in phase images than in height images. Phase images in tapping mode AFM show the phase difference between oscillation of the piezoelectric crystal that drives the cantilever and oscillation of the cantilever as it interacts with the sample surface. Phase images presented here show moving DNA molecules that have been replicated with Sequenase in the AFM and DNA molecules tethered in complexes with Escherichia coli RNA polymerase.
機(jī)譯:使用拍擊模式原子力顯微鏡(AFM)進(jìn)行相位成像具有許多優(yōu)勢(shì),可以以分子分辨率對(duì)水性緩沖液中的移動(dòng)DNA和DNA-酶復(fù)合物進(jìn)行成像。與來(lái)自AFM的高度圖像相比,相圖像中的分子可以以更高的掃描速率和更低的力進(jìn)行分辨。更高的掃描速率可以對(duì)更快的過(guò)程進(jìn)行成像。在較低的力下,分子的成像會(huì)更加柔和。與高度圖像相比,移動(dòng)的DNA分子在相位圖像中的分辨率也更高。敲擊模式AFM中的相位圖像顯示了驅(qū)動(dòng)懸臂的壓電晶體的振動(dòng)與懸臂與樣品表面相互作用時(shí)的振動(dòng)之間的相位差。此處顯示的相圖顯示了在AFM中已用Sequenase復(fù)制的移動(dòng)DNA分子,以及與大腸桿菌RNA聚合酶結(jié)合在一起的DNA分子。

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